7RHO
Human IgG1 Fc fragment, hinge-free, expressed in E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.03317 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.836, 150.392, 241.432 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.000 |
| R-factor | 0.2222 |
| Rwork | 0.220 |
| R-free | 0.27010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vgp |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.328 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 150.440 | 150.440 | 2.120 |
| High resolution limit [Å] | 2.000 | 6.350 | 2.010 |
| Rmerge | 0.083 | 0.034 | 1.739 |
| Rmeas | 0.088 | 0.036 | 1.831 |
| Rpim | 0.026 | 0.011 | 0.558 |
| Total number of observations | 43960 | 100172 | |
| Number of reflections | 114393 | 4473 | 10100 |
| <I/σ(I)> | 15.3 | 45.4 | 1.1 |
| Completeness [%] | 88.5 | 99.8 | 55.2 |
| Redundancy | 11 | 9.8 | 9.9 |
| CC(1/2) | 0.999 | 0.999 | 0.464 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 295 | 20% (w/v) PEG 6000, 40 mM CaCl2, 100 mM NaHepes |
