7RCM
Crystal Structure of ADP-bound Galactokinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-21 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 1.1533 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 128.793, 128.793, 240.652 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.820 - 2.100 |
| R-factor | 0.1975 |
| Rwork | 0.196 |
| R-free | 0.23560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wuu |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.680 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.820 | 39.760 | 2.150 |
| High resolution limit [Å] | 2.100 | 9.840 | 2.100 |
| Rmerge | 0.218 | 0.064 | 1.136 |
| Rmeas | 0.223 | 0.065 | 1.254 |
| Rpim | 0.046 | 0.011 | 0.515 |
| Total number of observations | 25394 | 22383 | |
| Number of reflections | 68728 | 810 | 4199 |
| <I/σ(I)> | 9.4 | 25.4 | 1.2 |
| Completeness [%] | 99.3 | 98.6 | 92 |
| Redundancy | 16.7 | 31.4 | 5.3 |
| CC(1/2) | 0.997 | 0.999 | 0.568 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 286 | Seeding into drops containing GALK protein at 8 mg/ml, 2.3M Na/K pH6.8 |
