7R96

Self-Assembled 3D DNA Hexagonal Tensegrity Triangle

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.60648
Spacegroup name	P 63
Unit cell lengths	125.206, 125.206, 65.227
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	54.220 - 5.680
Rwork	0.120
R-free	0.14300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gbi
RMSD bond length	0.005
RMSD bond angle	0.765
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	54.220	55.893	6.354
High resolution limit [Å]	5.680	11.831	5.683
Rmerge	0.187
Rmeas	0.193
Rpim	0.045
Number of reflections	1486	211	216
<I/σ(I)>	11.2		0.9
Completeness [%]	89.1	100	57.4
Redundancy	19.4	17.9	17.7
CC(1/2)	0.996	0.996	0.054

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	293	Ammonium sulfate, Tris, Acetic Acid, EDTA, Cobalt Hexammine