7R8W
Crystal Structure of the LNK SH2 Domain in Complex with a JAK2 pY813 Phosphopeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.060, 37.875, 38.883 |
| Unit cell angles | 90.00, 97.02, 90.00 |
Refinement procedure
| Resolution | 38.590 - 1.900 |
| R-factor | 0.2004 |
| Rwork | 0.195 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hdx |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.590 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.036 | 0.392 |
| Rmeas | 0.042 | 0.465 |
| Rpim | 0.023 | 0.248 |
| Number of reflections | 8093 | 798 |
| <I/σ(I)> | 18.7 | 2.84 |
| Completeness [%] | 99.6 | 98.88 |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.999 | 0.863 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 281.15 | 0.2 M ammonium chloride, 20% PEG3350 |
