7R86

Structure of mouse BAI1 (ADGRB1) in complex with mouse Nogo receptor (RTN4R)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	63.468, 95.315, 69.682
Unit cell angles	90.00, 105.39, 90.00

Refinement procedure

Resolution	38.871 - 1.650
R-factor	0.1656
Rwork	0.165
R-free	0.19450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ozn
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.240	40.240	1.680
High resolution limit [Å]	1.650	9.040	1.650
Rmerge	0.081	0.025	1.544
Rmeas	0.092	0.028	1.744
Rpim	0.042	0.013	0.795
Total number of observations	435598	2876	21390
Number of reflections	94133	606	4649
<I/σ(I)>	10.7	42.2	1
Completeness [%]	98.2	98.4	98.5
Redundancy	4.6	4.7	4.6
CC(1/2)	0.999	0.999	0.411

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	1 M succinic acid, pH 7.0, 0.02% w/v 4-Aminobenzioc acid, 0.02% w/v Azelaic acid, 0.02% w/v o-Sulfobenzoic acid monoammonium salt, 0.02% w/v Sodium 4-aminosalicylate dehydrate, 0.002 M HEPES sodium pH 6.8