7R71
Crystal Structure of the UbArk2C-UbcH5b~Ub complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.918400 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 53.330, 75.668, 99.379 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.591 - 2.800 |
| R-factor | 0.2305 |
| Rwork | 0.226 |
| R-free | 0.27140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v3l |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.553 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.6.2) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.990 | 46.990 | 2.950 |
| High resolution limit [Å] | 2.796 | 8.840 | 2.800 |
| Rmerge | 0.162 | 0.046 | 1.108 |
| Rmeas | 0.169 | 0.048 | 1.150 |
| Rpim | 0.046 | 0.014 | 0.307 |
| Total number of observations | 4155 | 20393 | |
| Number of reflections | 10462 | 388 | 1485 |
| <I/σ(I)> | 12.4 | 37.7 | 2.6 |
| Completeness [%] | 99.8 | 99.5 | 99 |
| Redundancy | 13.1 | 10.7 | 13.7 |
| CC(1/2) | 0.998 | 0.998 | 0.934 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 0.1 M MMT pH 6.0, 25% w/v PEG 1500 |
