7R6R

Crystal Structure of a Mycobacteriophage Cluster A2 Immunity Repressor:DNA Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.11608
Spacegroup name	C 1 2 1
Unit cell lengths	132.490, 43.510, 89.280
Unit cell angles	90.00, 102.26, 90.00

Refinement procedure

Resolution	47.390 - 3.130
R-factor	0.2125
Rwork	0.209
R-free	0.24760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7tz1
RMSD bond length	0.010
RMSD bond angle	1.599
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.390	3.240
High resolution limit [Å]	3.130	3.130
Number of reflections	9005	666
<I/σ(I)>	12.2
Completeness [%]	94.4
Redundancy	2
CC(1/2)	0.999	0.748

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	5-12% PEG 8,000, 0.1-0.3 M CaCl2, and 0.1M HEPES pH 7.5