7R3Y

The crystal structure of the V426L variant of Pol2CORE in complex with DNA and an incoming nucleotide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 1 2 1
Unit cell lengths	154.400, 70.100, 159.500
Unit cell angles	90.00, 113.10, 90.00

Refinement procedure

Resolution	47.840 - 2.600
R-factor	0.2227
Rwork	0.220
R-free	0.27020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdbid 4m8o
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.840	47.840	2.800
High resolution limit [Å]	2.600	15.000	2.600
Rmerge	0.075	0.020	1.494
Rmeas	0.089	0.024	1.774
Total number of observations	330071
Number of reflections	96815	545	19186
<I/σ(I)>	9.21	31.1	0.75
Completeness [%]	99.5	93.6	99.7
Redundancy	3.409	3.27	3.424
CC(1/2)	0.999	0.999	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	292	50mM MES pH6.5, PEG20K,2.5% glycerol, 150mM Sodium Actetate