7R3X
The crystal structure of the L439V variant of Pol2CORE in complex with DNA and an incoming nucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-29 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.976254 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 221.505, 70.685, 111.639 |
| Unit cell angles | 90.00, 100.14, 90.00 |
Refinement procedure
| Resolution | 45.780 - 2.460 |
| R-factor | 0.2119 |
| Rwork | 0.209 |
| R-free | 0.25620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4m8o |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.672 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.780 | 2.650 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmeas | 0.944 | 0.944 |
| Number of reflections | 61906 | 12304 |
| <I/σ(I)> | 7.95 | 0.97 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4.25 | 4.25 |
| CC(1/2) | 0.997 | 0.596 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 8 % PEG 20K , 150 mM NaAc, 0-1,5% Glycerol, 50 mM MES pH6.5 |
