7R2G
USP15 D1D2 in catalytically-competent state bound to mitoxantrone stack (isoform 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.882, 97.399, 62.995 |
| Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
| Resolution | 44.890 - 1.980 |
| R-factor | 0.2057 |
| Rwork | 0.204 |
| R-free | 0.23790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gha |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.510 |
| Data reduction software | XDS (VERSION Mar 15, 2019 BUILT=20190606) |
| Data scaling software | XDS (VERSION Mar 15, 2019 BUILT=20190606) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 97.400 | 44.860 | 2.030 |
| High resolution limit [Å] | 1.980 | 9.070 | 1.980 |
| Rmerge | 0.039 | 0.030 | 0.235 |
| Rmeas | 0.045 | 0.034 | 0.272 |
| Rpim | 0.023 | 0.017 | 0.134 |
| Total number of observations | 2195 | 13529 | |
| Number of reflections | 51964 | 576 | 3409 |
| <I/σ(I)> | 17.6 | 40.8 | 5.1 |
| Completeness [%] | 97.0 | 98.8 | 90.7 |
| Redundancy | 3.9 | 3.8 | 4 |
| CC(1/2) | 0.999 | 0.997 | 0.963 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | Sodium Hepes 0.1M, pH 7.0, PEG8000 5%. |
