7R23

Crystal structure of human Arc CTD in complex with two anti-Arc nanobodies

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	40.800, 61.640, 171.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.990 - 2.770
R-factor	0.2565
Rwork	0.252
R-free	0.30240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6tno 6tn7
RMSD bond length	0.002
RMSD bond angle	0.432
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.840
High resolution limit [Å]	2.770	2.770
Rmeas	0.251	3.407
Number of reflections	21261	849
<I/σ(I)>	7.5	0.6
Completeness [%]	99.6	99.9
Redundancy	6.6	6.1
CC(1/2)	0.995	0.324

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		281	20% PEG3350, 0.2 M Na malonate, 0.2 M KBr