7R1Z

C-terminal domain of hArc in complex with nanobodies H11 and C11, collapsed crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033
Spacegroup name	P 21 21 2
Unit cell lengths	66.260, 139.290, 43.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.000 - 1.940
R-factor	0.2005
Rwork	0.197
R-free	0.24250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6tnp
RMSD bond length	0.003
RMSD bond angle	0.608
Data reduction software	XDS (Feb 5, 2021)
Data scaling software	XSCALE (Feb 5, 2021)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (dev_3958)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	1.990
High resolution limit [Å]	1.940	1.940
Rmeas	0.130	2.356
Number of reflections	26403	1025
<I/σ(I)>	13.13	0.83
Completeness [%]	0.9	46.2
Redundancy	11.9	6.72
CC(1/2)	0.990	0.351

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	17% PEG3350. Produced from seed crystals grown in 12% PEG3350.