7QZQ
Crystal structure of the kelch domain of human KBTBD12
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-08-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.379, 55.078, 95.164 |
Unit cell angles | 90.00, 108.67, 90.00 |
Refinement procedure
Resolution | 45.080 - 1.880 |
R-factor | 0.1971 |
Rwork | 0.195 |
R-free | 0.23950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vpj |
Data reduction software | XDS (Feb 5, 2021 (BUILT 20210323)) |
Data scaling software | Aimless (0.7.7) |
Phasing software | PHASER (2.8.1) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.630 | 1.920 |
High resolution limit [Å] | 1.880 | 1.880 |
Rmerge | 0.156 | 3.100 |
Rmeas | 0.170 | |
Number of reflections | 57704 | 2827 |
<I/σ(I)> | 7.3 | 0.5 |
Completeness [%] | 99.9 | 97.7 |
Redundancy | 6.4 | 5.6 |
CC(1/2) | 0.997 | 0.268 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293.15 | 8% PEG4000 -- 0.1M acetate pH 4.5 |