7QUI
Crystal structure of the N-terminal domain of Siglec-8 in complex with sulfonamide sialoside analogue
Experimental procedure
| Experimental method | LAUE |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-25 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.71 - 2.25 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 91.682, 91.682, 150.131 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.336 - 3.352 |
| R-factor | 0.2662 |
| Rwork | 0.265 |
| R-free | 0.29210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7qu6 |
| Data reduction software | autoPROC |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.840 | 3.470 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.163 | 2.123 |
| Rpim | 0.037 | 0.476 |
| Number of reflections | 10954 | 1082 |
| <I/σ(I)> | 20.4 | 1.5 |
| Completeness [%] | 99.9 | |
| Redundancy | 2 | |
| CC(1/2) | 0.999 | 0.686 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.075 M Hepes pH 7.5, 15 % PEG 10000 and 25% glycerol |
