7QU6
Crystal structure of the N-terminal domain of Siglec-8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.075, 36.365, 167.516 |
| Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
| Resolution | 83.758 - 2.340 |
| R-factor | 0.2087 |
| Rwork | 0.206 |
| R-free | 0.25650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2n7a |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.820 | 2.420 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.179 | 1.249 |
| Rpim | 0.075 | 0.526 |
| Number of reflections | 36480 | 3624 |
| <I/σ(I)> | 10.8 | 2.6 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 6.7 | 6.1 |
| CC(1/2) | 0.990 | 0.630 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1 M Bis-Tris HCl pH 6.5, and 27% PEG 3350 |
