7QU6

Crystal structure of the N-terminal domain of Siglec-8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	70.075, 36.365, 167.516
Unit cell angles	90.00, 90.05, 90.00

Refinement procedure

Resolution	83.758 - 2.340
R-factor	0.2087
Rwork	0.206
R-free	0.25650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2n7a
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.820	2.420
High resolution limit [Å]	2.340	2.340
Rmerge	0.179	1.249
Rpim	0.075	0.526
Number of reflections	36480	3624
<I/σ(I)>	10.8	2.6
Completeness [%]	99.8	99.6
Redundancy	6.7	6.1
CC(1/2)	0.990	0.630

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.1 M Bis-Tris HCl pH 6.5, and 27% PEG 3350