7QU1
Machupo virus GP1 glycoprotein in complex with Fab fragment of antibody MAC1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-29 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 91.692, 100.731, 164.839 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.370 - 1.910 |
R-factor | 0.1962 |
Rwork | 0.194 |
R-free | 0.22940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5nuz |
RMSD bond length | 0.006 |
RMSD bond angle | 0.818 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.370 | 1.940 |
High resolution limit [Å] | 1.910 | 1.910 |
Rmerge | 0.118 | 1.515 |
Number of reflections | 59374 | 2930 |
<I/σ(I)> | 7.3 | |
Completeness [%] | 100.0 | 99.4 |
Redundancy | 6.8 | 6.6 |
CC(1/2) | 0.998 | 0.571 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 1.4 M sodium malonate (pH 6.0) |