7QQE
Nuclear factor one X - NFIX in P41212
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.28199 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 65.009, 65.009, 127.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.590 - 3.500 |
| R-factor | 0.2247 |
| Rwork | 0.223 |
| R-free | 0.25890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7qqd |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.031 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.970 | 3.850 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rpim | 0.116 | 0.919 |
| Number of reflections | 3802 | 366 |
| <I/σ(I)> | 7.6 | 1.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 25.1 | 26.6 |
| CC(1/2) | 0.998 | 0.603 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M NaSCN, 20% PEG 3350 |
