7QP4

Complex of a Gemini-cholesterol analogue with Retinoid-related Orphan Receptor gamma

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-25
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.979957
Spacegroup name	P 61
Unit cell lengths	99.381, 99.381, 129.308
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.830 - 2.300
R-factor	0.18
Rwork	0.178
R-free	0.23730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3l0l
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.830	2.382
High resolution limit [Å]	2.300	2.300
Number of reflections	32145	3197
<I/σ(I)>	8.18
Completeness [%]	99.2
Redundancy	9.7
CC(1/2)	0.970	0.124

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.8M NH4 acetate, 0.1M Hepes pH = 7.5