7QIV

Structure of human C3b in complex with the EWE nanobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-07
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 2
Unit cell lengths	255.310, 64.880, 144.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.310 - 2.800
R-factor	0.2364
Rwork	0.235
R-free	0.27480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ehg
RMSD bond length	0.004
RMSD bond angle	0.730
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.310	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.224	2.725
Rmeas	0.233
Number of reflections	60284	5282
<I/σ(I)>	11.74	0.73
Completeness [%]	98.6	89.1
Redundancy	13.5	14.1
CC(1/2)	0.998	0.495

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	292	112 mM Sodium Citrate pH 8, 28 mM Sodium Citrate pH 6.0, 12.25 % (w/v) PEG 2000 MME, 70 mM Imidazole pH 7, 60 mM Ammonium acetate, 70 mM TRIS pH 8.5, and 13.5 % (v/v) 2-Methyl-2,4-pentanediol