7QI1
Crystal structure of human 14-3-3 protein beta in complex with CFTR peptide pS753pS768 and PPI stabilizer CY007424
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2017-05-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 70.367, 111.460, 130.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.280 - 1.760 |
| R-factor | 0.1975 |
| Rwork | 0.195 |
| R-free | 0.24110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6hep |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.788 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BUSTER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.282 | 1.970 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Number of reflections | 102027 | 4934 |
| <I/σ(I)> | 20 | |
| Completeness [%] | 99.9 | |
| Redundancy | 12.9 | |
| CC(1/2) | 1.000 | 0.796 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | Qiagen Cryos Suite #44 (0.09 M HEPES sodium salt pH7.5, 1.26M tri-sodium citrate, 10% (v/v) glycerol) with an extra 2% (v/v) of glycerol. |
