7QGP
STK10 (LOK) bound to Macrocycle CKJB51
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-05-15 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.000041 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.312, 61.839, 56.808 |
| Unit cell angles | 90.00, 102.79, 90.00 |
Refinement procedure
| Resolution | 40.180 - 1.900 |
| R-factor | 0.2013 |
| Rwork | 0.200 |
| R-free | 0.23680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6eim |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.318 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.180 | 40.150 | 1.940 |
| High resolution limit [Å] | 1.900 | 9.110 | 1.900 |
| Rmerge | 0.043 | 0.031 | 0.237 |
| Rmeas | 0.050 | 0.036 | 0.275 |
| Rpim | 0.025 | 0.018 | 0.138 |
| Total number of observations | 892 | 6357 | |
| Number of reflections | 24697 | 241 | 1600 |
| <I/σ(I)> | 11.6 | 19.9 | 3.6 |
| Completeness [%] | 99.1 | 98.4 | 99.6 |
| Redundancy | 3.8 | 3.7 | 4 |
| CC(1/2) | 0.998 | 0.998 | 0.967 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 13% PEG 1k, 10% PEG 8k |
