7QGI

Crystal structure of SARS-CoV-2 NSP14 in the absence of NSP10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9179
Spacegroup name	P 21 21 21
Unit cell lengths	67.539, 68.093, 138.428
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.100 - 1.650
R-factor	0.1968
Rwork	0.196
R-free	0.22070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5c8s
RMSD bond length	0.002
RMSD bond angle	0.507
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.214	1.850
High resolution limit [Å]	1.650	1.650
Rmerge	0.125	1.075
Rpim	0.059	0.466
Number of reflections	37654	1884
<I/σ(I)>	9.2	1.6
Completeness [%]	89.2	65.6
Redundancy	5.5	6.2
CC(1/2)	0.997	0.716

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	1.26 M sodium phosphate monobasic, 0.14 M potassium phosphate dibasic