7QG6
Co-crystal structure of UPF3A-RRM-NOPS-L with UPF2-MIF4GIII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 277 |
| Detector technology | PIXEL |
| Collection date | 2021-06-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.677, 108.579, 119.943 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 65.250 - 2.950 |
| R-factor | 0.2096 |
| Rwork | 0.207 |
| R-free | 0.26320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uw4 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.250 | 3.020 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rpim | 0.087 | 0.500 |
| Number of reflections | 41731 | 2660 |
| <I/σ(I)> | 7.2 | 2.1 |
| Completeness [%] | 99.2 | 99.8 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 0.1 M Sodium HEPES pH 7.0 10% w/v PEG 4000 10% v/v 2-Propanol |
