7QF9

Crystal structure of PDE6D bound to HRas peptide

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	34.626, 82.789, 112.467
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.234 - 1.950
Rwork	0.230
R-free	0.29930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tb5
RMSD bond length	0.002
RMSD bond angle	1.165
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.234	2.000
High resolution limit [Å]	1.950	1.950
Number of reflections	24377	1768
<I/σ(I)>	16.9
Completeness [%]	99.8
Redundancy	6.4
CC(1/2)	1.000	0.772

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	0.1 M Tris pH 8.5, 8% w/v PEG 8000