7QEZ
Crystal structure of the SARS-CoV-2 RBD in complex with the ultrapotent antibody CV2.1169 and CR3022
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2021-06-01 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.98011 |
Spacegroup name | P 41 |
Unit cell lengths | 97.751, 97.751, 164.474 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.880 | 48.880 | 3.020 |
High resolution limit [Å] | 2.880 | 9.560 | 2.880 |
Rmerge | 0.261 | 0.062 | 3.509 |
Rmeas | 0.271 | 0.064 | 3.643 |
Rpim | 0.072 | 0.018 | 0.971 |
Total number of observations | 485839 | 12934 | 61055 |
Number of reflections | 34600 | 986 | 4402 |
<I/σ(I)> | 10.2 | 36.8 | 0.9 |
Completeness [%] | 99.4 | 99.2 | 96 |
Redundancy | 14 | 13.1 | 13.9 |
CC(1/2) | 0.995 | 0.996 | 0.214 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 15% PEG 8000, 0.5 M lithium sulfate |