7QEM
bacterial IMPDH chimera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 4 |
| Unit cell lengths | 143.320, 143.320, 119.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.770 - 3.090 |
| R-factor | 0.2029 |
| Rwork | 0.199 |
| R-free | 0.24120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dqw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.296 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.770 | 3.150 |
| High resolution limit [Å] | 3.070 | 3.070 |
| Rmerge | 0.415 | 2.788 |
| Rmeas | 0.432 | 2.914 |
| Rpim | 0.119 | 0.832 |
| Number of reflections | 44463 | 2450 |
| <I/σ(I)> | 6 | 1 |
| Completeness [%] | 98.0 | 73.4 |
| Redundancy | 13.1 | 11.2 |
| CC(1/2) | 0.981 | 0.314 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 1%w/v Tryptone, 0.05 M HEPES 7 pH, 12%w/v PEG 3350, 0.001% w/V NaN3 |
