7QEI

Structure of human MTHFD2L in complex with TH7299

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-17
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	77.110, 109.264, 73.025
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.750 - 2.100
R-factor	0.18966
Rwork	0.187
R-free	0.23110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tc4
RMSD bond length	0.011
RMSD bond angle	1.521
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.700	2.160
High resolution limit [Å]	2.100	2.100
Number of reflections	18319	1478
<I/σ(I)>	6.7
Completeness [%]	99.9
Redundancy	7.3
CC(1/2)	0.985	0.413

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M NaF, 20 % PEG3350