7QBU

B12-dependent radical SAM methyltransferase, Mmp10 with [4Fe-4S] cluster, cobalamin, and S-methyl-5'-thioadenosine bound.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	77.779, 79.493, 151.659
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.840 - 2.298
R-factor	0.1927
Rwork	0.191
R-free	0.22470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7qbs
RMSD bond length	0.008
RMSD bond angle	1.210
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	REFMAC
Refinement software	BUSTER (2.10.4 (16-JUL-2021))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.840	2.360
High resolution limit [Å]	2.298	2.300
Rmerge	0.119
Rpim	0.034	0.907
Number of reflections	37624	753
<I/σ(I)>	14.8	0.8
Completeness [%]	99.7	96.1
Redundancy	12.8	7.8
CC(1/2)	0.999	0.315

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	20% (w/v) PEG8000, 100 mM Tris