7QBE

TC:CD320 in complex with nanobody TC-Nb11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	103.717, 195.831, 198.385
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.960 - 3.000
R-factor	0.2539
Rwork	0.252
R-free	0.28370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zrp
RMSD bond length	0.013
RMSD bond angle	2.209
Data reduction software	X-Area
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.970	3.107
High resolution limit [Å]	3.000	3.000
Number of reflections	81479	8030
<I/σ(I)>	11.45
Completeness [%]	99.8
Redundancy	2
CC(1/2)	0.997	0.791

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298.15	0.2 M sodium malonate pH 6.0, 20% w/v PEG 3350