7QAR

Serial crystallography structure of cofactor-free urate oxidase in complex with the 5-peroxo derivative of 9-methyl uric acid at room temperature

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	293
Detector technology	PIXEL
Collection date	2019-04-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03
Spacegroup name	I 2 2 2
Unit cell lengths	79.790, 96.030, 105.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.070 - 2.300
Rwork	0.218
R-free	0.26610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cw2
RMSD bond length	0.008
RMSD bond angle	1.388
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.070	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	18280	1796
<I/σ(I)>	2.44	0.52
Completeness [%]	100.0	100
Redundancy	28.2	20.6
CC(1/2)	0.849	0.225

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7.6	291	TRIS-ACETATE, PEG 8000, MUA,