7QAA

Crystal structure of RARalpha/RXRalpha ligand binding domain heterodimer in complex with BMS614 and oleic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	120
Detector technology	CCD
Collection date	1997-12-05
Detector	MAR CCD 130 mm
Wavelength(s)	0.918
Spacegroup name	P 61 2 2
Unit cell lengths	116.600, 116.600, 207.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.820 - 2.760
R-factor	0.1971
Rwork	0.195
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dkf
RMSD bond length	0.005
RMSD bond angle	0.987
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.470	2.860
High resolution limit [Å]	2.760	2.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	23% PEG 10,000 0.1 M Hepes, pH 7.25