7Q9Q
Crystal structure of PDE6D Geranylgeranylated cystein complex
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-25 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.048, 35.289, 37.645 |
| Unit cell angles | 77.72, 68.98, 69.23 |
Refinement procedure
| Resolution | 34.971 - 1.450 |
| Rwork | 0.225 |
| R-free | 0.27360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tb5 |
| RMSD bond length | 0.000 |
| RMSD bond angle | 1.839 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.971 | 1.490 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 24436 | 1715 |
| <I/σ(I)> | 17.98 | |
| Completeness [%] | 95.9 | |
| Redundancy | 3.5 | |
| CC(1/2) | 1.000 | 0.669 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 30% w/v PEG 1500 |
