7Q5O

Bromodomain-containing 2 BD2 in complex with the inhibitor CRCM5484

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	275
Detector technology	PIXEL
Collection date	2020-10-28
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.885602
Spacegroup name	P 21 21 2
Unit cell lengths	52.430, 72.494, 32.041
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.816 - 1.519
R-factor	0.2176
Rwork	0.216
R-free	0.24640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xhk
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.820	1.574
High resolution limit [Å]	1.519	1.520
Rmerge	0.180	0.905
Rmeas	0.207	1.059
Rpim	0.101	0.535
Number of reflections	19199	1857
<I/σ(I)>	4.71
Completeness [%]	98.3
Redundancy	3.9
CC(1/2)	0.927	0.081

Crystallization Conditions

crystal ID	method	pH	temperature	details
1