7Q41

Crystal structure of RCC1-Like domain 2 of ubiquitin ligase HERC2 in complex with DXDKDED motif of ubiquitin-protein ligase E3A (E6AP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-28
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.999882
Spacegroup name	P 43 21 2
Unit cell lengths	108.952, 108.952, 243.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.725 - 3.015
R-factor	0.201297269544
Rwork	0.198
R-free	0.25505
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kci
RMSD bond length	0.009
RMSD bond angle	1.094
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.725	3.200
High resolution limit [Å]	3.010	3.010
Number of reflections	54825	8710
<I/σ(I)>	5.81
Completeness [%]	99.2
Redundancy	5.91558595531
CC(1/2)	0.968	0.401

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293.15	293.1500 hanging drop 20% PEG 3350 0.18M tris amonium citrate