7Q00
Crystal structure of serine hydroxymethyltransferase, isoform 4 from Arabidopsis thaliana (SHM4)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.091, 130.914, 121.778 |
Unit cell angles | 90.00, 92.26, 90.00 |
Refinement procedure
Resolution | 49.890 - 1.740 |
R-factor | 0.1618 |
Rwork | 0.162 |
R-free | 0.20140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6smr |
RMSD bond length | 0.008 |
RMSD bond angle | 0.980 |
Data reduction software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 1.840 |
High resolution limit [Å] | 1.740 | 1.740 |
Rmerge | 0.077 | 0.592 |
Number of reflections | 181530 | 29467 |
<I/σ(I)> | 8.62 | 1.99 |
Completeness [%] | 95.5 | |
Redundancy | 2.7 | 2.6 |
CC(1/2) | 0.994 | 0.645 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292 | D9 Index (Hampton Research); 0.1 M Tris 8.5, 25% w/v, Polyethylene glycol 3,350 cryoprotection with the reservoir condition supplemented with 20% ethylene glycol |