7PZZ
Crystal structure of serine hydroxymethyltransferase, isoform 2 from Arabidopsis thaliana (SHM2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-09-04 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 115.201, 131.236, 151.389 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.840 - 1.650 |
R-factor | 0.158 |
Rwork | 0.158 |
R-free | 0.17790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6smw |
RMSD bond length | 0.008 |
RMSD bond angle | 1.008 |
Data reduction software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 1.750 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.094 | 1.140 |
Number of reflections | 274997 | 43063 |
<I/σ(I)> | 17.19 | 1.85 |
Completeness [%] | 99.5 | 97 |
Redundancy | 8 | 7.3 |
CC(1/2) | 0.999 | 0.650 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | SHM2 15 mg/ml; drop 3+2 uL 90% Index (Hampton Research) F8 (0.2 M Ammonium sulfate, 0.1 M HEPES 7.5, 25% w/v Polyethylene glycol 3,350) cryoprotection with 20% ethylene glycol in the F8 Index condition |