7PWD
Structure of an inhibited GRK2-G-beta and G-gamma complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 185.600, 74.549, 123.411 |
| Unit cell angles | 90.00, 115.31, 90.00 |
Refinement procedure
| Resolution | 111.560 - 2.600 |
| R-factor | 0.1924 |
| Rwork | 0.192 |
| R-free | 0.22950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.191 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 111.560 | 2.850 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.044 | 0.434 |
| Rmeas | 0.053 | 0.508 |
| Number of reflections | 46426 | 11050 |
| <I/σ(I)> | 16.24 | 3.5 |
| Completeness [%] | 98.2 | 97.8 |
| Redundancy | 3.6 | 3.7 |
| CC(1/2) | 0.999 | 0.926 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | ethylene glycol, PEG 3350, MES, NaCl |
