7PUX

Structure of p97 N-D1(L198W) in complex with Fragment TROLL2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9184
Spacegroup name	P 6 2 2
Unit cell lengths	146.234, 146.234, 84.397
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.870 - 1.730
R-factor	0.1942
Rwork	0.192
R-free	0.23530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dyg
RMSD bond length	0.006
RMSD bond angle	0.885
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.870	1.830
High resolution limit [Å]	1.730	1.730
Rmerge	0.137	2.498
Rmeas	0.140	2.547
Rpim	0.027	0.492
Number of reflections	42850	2143
<I/σ(I)>	16.684	1.63
Completeness [%]	96.1	25.31
Redundancy	26.47
CC(1/2)	0.999	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293.15	Natriumformiat (pH 6.0) 4.0 M Glycerol (v/v) 10 % PEG 600 (v/v) 5 %