7PU5
Structure of SFPQ-NONO complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 466.999, 66.820, 126.893 |
| Unit cell angles | 90.00, 105.89, 90.00 |
Refinement procedure
| Resolution | 48.450 - 2.999 |
| R-factor | 0.2246 |
| Rwork | 0.223 |
| R-free | 0.25520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ifm |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.860 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4 (16-JUL-2021)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.450 | 3.200 |
| High resolution limit [Å] | 2.999 | 3.000 |
| Rmerge | 0.130 | |
| Rpim | 0.069 | 0.588 |
| Number of reflections | 259406 | 2967 |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 93.9 | |
| Redundancy | 4.4 | |
| CC(1/2) | 0.998 | 0.569 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 25 mM Tris-HCl pH8.5, 14-16 % PEG 8K, 20 % w/v |
