7PS9

Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with Iberdomide (CC-220)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9999
Spacegroup name	P 21 21 21
Unit cell lengths	56.001, 58.850, 88.583
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.760 - 1.800
R-factor	0.1956
Rwork	0.193
R-free	0.24620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v2y
RMSD bond length	0.008
RMSD bond angle	1.546
Data reduction software	XDS
Data scaling software	XDS
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0266)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.760	1.910
High resolution limit [Å]	1.800	1.800
Rmerge	0.135	1.542
Rmeas	0.141	1.611
Number of reflections	27318	4220
<I/σ(I)>	11.24	1.21
Completeness [%]	97.7	94.9
Redundancy	12.5	11
CC(1/2)	0.998	0.845

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.5 M (NH4)H2PO4