7PPC
Ternary signalling complex of BMP10 bound to ALK1 and BMPRII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-26 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.91188 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.761, 130.387, 88.825 |
| Unit cell angles | 90.00, 103.38, 90.00 |
Refinement procedure
| Resolution | 86.410 - 3.600 |
| R-factor | 0.2455 |
| Rwork | 0.244 |
| R-free | 0.28250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6sf3 7ppa |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.622 |
| Data reduction software | DIALS (1.14.13) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 130.390 | 3.940 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.331 | 1.223 |
| Rmeas | 0.371 | 1.369 |
| Rpim | 0.163 | 0.604 |
| Number of reflections | 11220 | 789 |
| <I/σ(I)> | 2.5 | 0.9 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 4.9 | 4.9 |
| CC(1/2) | 0.977 | 0.673 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294.15 | 20% PEG 3350 0.2 M Calcium acetate hydrate |
