7PLI
DEAD-box helicase DbpA bound to single stranded RNA and ADP/BeF3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-08-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9766 |
Spacegroup name | P 1 |
Unit cell lengths | 58.643, 121.599, 122.417 |
Unit cell angles | 97.16, 92.43, 103.44 |
Refinement procedure
Resolution | 56.880 - 2.500 |
R-factor | 0.2149 |
Rwork | 0.213 |
R-free | 0.24690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2db3 3moj |
RMSD bond length | 0.004 |
RMSD bond angle | 0.834 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (1.18.2_3874+SVN) |
Refinement software | PHENIX (1.18.2_3874+SVN) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.880 | 2.589 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 108844 | 10869 |
<I/σ(I)> | 7.25 | 1.75 |
Completeness [%] | 96.6 | 96.52 |
Redundancy | 3.6 | 3.6 |
CC(1/2) | 0.990 | 0.750 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20 % (v/v) PEG 3350, 400 mM KSCN, 100 mM Tris, pH 8.0 |