7PKL
Mechanistic understanding of antibody masking with anti-idiotypic antibody fragments
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 160.742, 59.965, 63.499 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.370 - 2.350 |
| R-factor | 0.184 |
| Rwork | 0.180 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hjg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.260 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 80.371 | 80.371 | 2.592 |
| High resolution limit [Å] | 2.345 | 7.855 | 2.345 |
| Rmerge | 0.303 | 0.093 | 1.247 |
| Rmeas | 0.328 | 0.102 | 1.366 |
| Rpim | 0.126 | 0.042 | 0.546 |
| Total number of observations | 105869 | 4547 | 4526 |
| Number of reflections | 15991 | 799 | 800 |
| <I/σ(I)> | 5.7 | 12.8 | 1.4 |
| Completeness [%] | 93.0 | 99.5 | 55.1 |
| Redundancy | 6.6 | 5.7 | 5.7 |
| CC(1/2) | 0.979 | 0.995 | 0.567 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | Peg4000 25%, Ammonium Sulphate 0.2, Sodium Acetate 0.1M, pH 4.6 |
