7PJH

Crystal structure of the human spliceosomal maturation factor AAR2 bound to the RNAse H domain of PRPF8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.2395
Spacegroup name	C 1 2 1
Unit cell lengths	145.280, 57.260, 111.230
Unit cell angles	90.00, 112.74, 90.00

Refinement procedure

Resolution	48.210 - 2.350
R-factor	0.1912
Rwork	0.189
R-free	0.23480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3e9l
RMSD bond length	0.009
RMSD bond angle	0.990
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.233	2.410
High resolution limit [Å]	2.350	2.350
Number of reflections	34687	2502
<I/σ(I)>	1.7
Completeness [%]	97.6
Redundancy	3.79
CC(1/2)	0.990	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M HEPES pH 7.0, 10% PEG 6000, 5%(v/v) 2-methyl-2,4- pentanediol