7PGA
Chimeric carminomycin-4-O-methyltransferase (DnrK) with regions from 10-hydroxylase RdmB and 10-decarboxylase TamK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-01 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9679 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.470, 102.640, 121.850 |
| Unit cell angles | 90.00, 98.23, 90.00 |
Refinement procedure
| Resolution | 45.890 - 2.770 |
| R-factor | 0.2034 |
| Rwork | 0.201 |
| R-free | 0.25330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tw2 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.892 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.800 | 9.220 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.127 | 0.963 |
| Number of reflections | 58153 | 5430 |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 98.4 | |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293.15 | 0.1 M Sodium acetate, pH 4.6; 8% PEG 8000 |
