7PGA

Chimeric carminomycin-4-O-methyltransferase (DnrK) with regions from 10-hydroxylase RdmB and 10-decarboxylase TamK

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Carminomycin 4-O-methyltransferase DnrK,Methyltransferase domain-containing protein,Aclacinomycin 10-hydroxylase RdmB	polymer	368	40000.4	4	UniProt (Q06528) UniProt (I2N5E8) UniProt (Q54527)	Streptomyces peucetius	COMT,Anthracycline 4-O-methyltransferase,O-methyltransferase,15-demethoxy-epsilon-rhodomycin 10-hydroxylase,15-demethoxyaclacinomycin T
2	E, G, I, K (A, B, C, D)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	4	Chemie (SAH)
3	F, H, J, L (A, B, C, D)	methyl (1R,2R,4S)-2-ethyl-2,4,5,7-tetrahydroxy-6,11-dioxo-1,2,3,4,6,11-hexahydrotetracene-1-carboxylate	non-polymer		412.4	4	Chemie (VAK)
4	M, N, O, P (A, B, C, D)	water	water		18.0	141	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 159 (UniProt: Q06528)

PDB	External Database	Details
Met -9	-	initiating methionine
Ala -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Arg 0	-	expression tag
Ser 1	-	expression tag

A, B, C, D: 160 - 169 (UniProt: I2N5E8)
A, B, C, D: 170 - 285 (UniProt: Q06528)
A, B, C, D: 286 - 300 (UniProt: Q54527)
A, B, C, D: 301 - 334 (UniProt: Q06528)
A, B, C, D: 335 - 347 (UniProt: I2N5E8)
A, B, C, D: 348 - 358 (UniProt: Q06528)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	160001.8
Non-Polymers*	Number of molecules	8
	Total formula weight	3187.2
All*	Total formula weight	163189.0

*Water molecules are not included.