7PDX

Crystal structure of parent MAGE-A10 TCR (728)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 1
Unit cell lengths	54.930, 59.171, 86.020
Unit cell angles	101.94, 105.87, 104.69

Refinement procedure

Resolution	79.010 - 2.270
Rwork	0.192
R-free	0.25160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7pdw
RMSD bond length	0.009
RMSD bond angle	1.560
Data reduction software	MOSFLM
Data scaling software	Aimless (0.7.2)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	79.011	79.010	2.340
High resolution limit [Å]	2.270	9.080	2.270
Rmerge	0.117	0.072	0.210
Rmeas	0.166	0.101	0.297
Rpim	0.117	0.072	0.210
Number of reflections	38348	657	3471
<I/σ(I)>	4.3	6.1	2.3
Completeness [%]	86.2	94.4	85
Redundancy	1.5	1.6	1.5
CC(1/2)	0.957	0.984	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293.15	0.1M PCTP pH 7.0, 25% w/v PEG 1500