7PAC
The crystal structure of PDE6D in the apo state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.250, 39.180, 44.680 |
| Unit cell angles | 90.00, 105.89, 90.00 |
Refinement procedure
| Resolution | 42.970 - 2.050 |
| R-factor | 0.1879 |
| Rwork | 0.185 |
| R-free | 0.23240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nal |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.496 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | DIMPLE |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.970 | 2.110 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.108 | 0.533 |
| Rmeas | 0.150 | 0.735 |
| Rpim | 0.105 | |
| Number of reflections | 9410 | 2165 |
| <I/σ(I)> | 5.7 | 2 |
| Completeness [%] | 98.8 | 98.2 |
| Redundancy | 3 | 3.1 |
| CC(1/2) | 0.961 | 0.556 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 295 | 0.1M Hepes pH7.4, 20 mM MgCl2, 20 mM NiCl2, 20% PEG3350 |
