7PA7

BK polyomavirus VP1 in complex with scFv 29B1

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	93
Detector technology	PIXEL
Collection date	2017-10-13
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.99
Spacegroup name	P 1
Unit cell lengths	114.588, 114.614, 142.373
Unit cell angles	92.31, 106.00, 111.41

Refinement procedure

Resolution	48.444 - 2.600
Rwork	0.247
R-free	0.27240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mj1 4kq3
RMSD bond length	0.007
RMSD bond angle	1.438
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.200	2.810
High resolution limit [Å]	2.600	2.600
Rmeas	0.170	0.740
Number of reflections	181005	55527
<I/σ(I)>	5.9	1.4
Completeness [%]	94.8
Redundancy	1.8
CC(1/2)	0.980	0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.1 M sodium malonate (pH 5.5), 14.4% PEG 3350