Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-02 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97996 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 66.743, 66.743, 176.785 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 57.802 - 1.420 |
| Rwork | 0.174 |
| R-free | 0.21380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6m8z |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.985 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.930 | 1.440 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rpim | 0.033 | 1.416 |
| Number of reflections | 44987 | 2131 |
| <I/σ(I)> | 17.5 | 0.8 |
| Completeness [%] | 100.0 | 98.9 |
| Redundancy | 32.5 | 9.1 |
| CC(1/2) | 1.000 | 0.335 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M Ammonium sulfate 0.1 M Bis-Tris pH 5.5 25% (w/v) PEG 3350 |
